{"id":14130,"date":"2019-12-19T14:49:10","date_gmt":"2019-12-19T06:49:10","guid":{"rendered":"https:\/\/id-ea.org\/tbe\/?p=14130"},"modified":"2019-12-23T16:46:55","modified_gmt":"2019-12-23T08:46:55","slug":"snapshot-week-52-2019-japanese-tick-borne-encephalitis-cross-reactivity","status":"publish","type":"post","link":"https:\/\/tbenews.com\/tbe\/snapshot-week-52-2019-japanese-tick-borne-encephalitis-cross-reactivity\/","title":{"rendered":"Snapshot week 52\/2019<br> Japanese\/tick-borne encephalitis cross-reactivity"},"content":{"rendered":"<span class=\"cb-itemprop\" itemprop=\"reviewBody\">\n<p class=\"wp-block-paragraph\">Maeki et al.<br>Analysis of cross-reactivity between flaviviruses with sera of patients with Japanese encephalitis showed the importance of neutralization tests for diagnosis of Japanese encephalitis<br><em>J. Infect. Chemother.<\/em> 2019, 25: 786-790<br><br>Japanese encephalitis (JE) is the most important viral encephalitis in Asia and is usually diagnosed by serological assays. Antibodies between flaviviruses have been reported to show cross-reactivity, and this is in particular true for ELISAs (see Snapshots of <a href=\"https:\/\/id-ea.org\/tbe\/snapshot-week-12-2019-comparison-of-four-commercial-tbe-elisas\/\">week 12<\/a>, <a href=\"https:\/\/id-ea.org\/tbe\/snapshot-week-18-2019-how-to-discriminate-between-tbe-and-west-nile-virus-infections\/\">18<\/a> and <a href=\"https:\/\/id-ea.org\/tbe\/snapshot-week-22-2019-recombinant-glycoprotein-e-of-tbe-virus-for-diagnostic-purposes\/\">22\/2019<\/a>). A detailed analysis has been carried out using 16 serum samples from collected 10 patients with JE reported in Japan. These patients were diagnosed by detection of IgM using IgM-capture ELISA and by neutralization test. In this study, one serum sample showed a positive reaction in TBE IgM-ELISA and eight sera were positive in an IgG-ELISA. Cross-reactivity was also shown with sera from West Nile virus and Dengue virus. However, in a neutralization test, all sera were specific for JE and showed no positive cross-reactivity with TBE virus.<br><br>TBE is prevalent in some parts of Japan, and it has also been shown that sera from TBE patients have cross-reactivity with JEV. Therefore, differential diagnosis must be carried out by neutralization test. <\/p>\n<\/span>","protected":false},"excerpt":{"rendered":"<p>Maeki et al.Analysis of cross-reactivity between flaviviruses with sera of patients with Japanese encephalitis showed the importance of neutralization tests for diagnosis of Japanese encephalitisJ. Infect. Chemother. 2019, 25: 786-790 Japanese encephalitis (JE) is the most important viral encephalitis in Asia and is usually diagnosed by serological assays. Antibodies between flaviviruses have been reported to show cross-reactivity, and this is in particular true for ELISAs (see Snapshots of week 12, 18 and 22\/2019). A detailed analysis has been carried out using 16 serum samples from collected 10 patients with JE reported in Japan. These patients were diagnosed by detection of IgM using IgM-capture ELISA and by neutralization test. In this study, one serum sample showed a positive reaction in TBE IgM-ELISA and eight sera were positive in an IgG-ELISA. Cross-reactivity was also shown with sera from West Nile virus and Dengue virus. However, in a neutralization test, all sera were specific for JE and showed no positive cross-reactivity with TBE virus. TBE is prevalent in some parts of Japan, and it has also been shown that sera from TBE patients have cross-reactivity with JEV. Therefore, differential diagnosis must be carried out by neutralization test.<\/p>\n","protected":false},"author":6,"featured_media":12864,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"footnotes":""},"categories":[18],"tags":[223,214,245,363,362],"coauthors":[81],"class_list":["post-14130","post","type-post","status-publish","format-standard","has-post-thumbnail","category-snapshot","tag-cross-reactivity","tag-elisa","tag-flaviviruses","tag-japanese-encephalitis-virus","tag-neutralization-test"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Snapshot week 52\/2019 Japanese\/tick-borne encephalitis cross-reactivity - TBE Book<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/tbenews.com\/tbe\/snapshot-week-52-2019-japanese-tick-borne-encephalitis-cross-reactivity\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Snapshot week 52\/2019 Japanese\/tick-borne encephalitis cross-reactivity - TBE Book\" \/>\n<meta property=\"og:description\" content=\"Maeki et al.Analysis of cross-reactivity between flaviviruses with sera of patients with Japanese encephalitis showed the importance of neutralization tests for diagnosis of Japanese encephalitisJ. Infect. Chemother. 2019, 25: 786-790 Japanese encephalitis (JE) is the most important viral encephalitis in Asia and is usually diagnosed by serological assays. Antibodies between flaviviruses have been reported to show cross-reactivity, and this is in particular true for ELISAs (see Snapshots of week 12, 18 and 22\/2019). A detailed analysis has been carried out using 16 serum samples from collected 10 patients with JE reported in Japan. These patients were diagnosed by detection of IgM using IgM-capture ELISA and by neutralization test. In this study, one serum sample showed a positive reaction in TBE IgM-ELISA and eight sera were positive in an IgG-ELISA. Cross-reactivity was also shown with sera from West Nile virus and Dengue virus. However, in a neutralization test, all sera were specific for JE and showed no positive cross-reactivity with TBE virus. TBE is prevalent in some parts of Japan, and it has also been shown that sera from TBE patients have cross-reactivity with JEV. Therefore, differential diagnosis must be carried out by neutralization test.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/tbenews.com\/tbe\/snapshot-week-52-2019-japanese-tick-borne-encephalitis-cross-reactivity\/\" \/>\n<meta property=\"og:site_name\" content=\"TBE Book\" \/>\n<meta property=\"article:published_time\" content=\"2019-12-19T06:49:10+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2019-12-23T08:46:55+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/tbenews.com\/tbe\/wp-content\/uploads\/2017\/11\/TBE-Snapshots.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1180\" \/>\n\t<meta property=\"og:image:height\" content=\"250\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Michael Br\u00f6ker\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Michael Br\u00f6ker\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-52-2019-japanese-tick-borne-encephalitis-cross-reactivity\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-52-2019-japanese-tick-borne-encephalitis-cross-reactivity\\\/\"},\"author\":{\"name\":\"augustine\",\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/#\\\/schema\\\/person\\\/2f903afeb7534df7457d2e3f6d30f64c\"},\"headline\":\"Snapshot week 52\\\/2019 Japanese\\\/tick-borne encephalitis cross-reactivity\",\"datePublished\":\"2019-12-19T06:49:10+00:00\",\"dateModified\":\"2019-12-23T08:46:55+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-52-2019-japanese-tick-borne-encephalitis-cross-reactivity\\\/\"},\"wordCount\":195,\"publisher\":{\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-52-2019-japanese-tick-borne-encephalitis-cross-reactivity\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/wp-content\\\/uploads\\\/2017\\\/11\\\/TBE-Snapshots.jpg\",\"keywords\":[\"cross-reactivity\",\"ELISA\",\"flaviviruses\",\"Japanese encephalitis virus\",\"neutralization test\"],\"articleSection\":[\"Snapshot\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-52-2019-japanese-tick-borne-encephalitis-cross-reactivity\\\/\",\"url\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-52-2019-japanese-tick-borne-encephalitis-cross-reactivity\\\/\",\"name\":\"Snapshot week 52\\\/2019 Japanese\\\/tick-borne encephalitis cross-reactivity - 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