{"id":19576,"date":"2022-06-06T12:52:50","date_gmt":"2022-06-06T04:52:50","guid":{"rendered":"https:\/\/tbenews.com\/tbe\/?p=19576"},"modified":"2022-06-06T12:52:51","modified_gmt":"2022-06-06T04:52:51","slug":"snapshot-week-23-2022-detection-of-tbe-virus-in-dairy-products","status":"publish","type":"post","link":"https:\/\/tbenews.com\/tbe\/snapshot-week-23-2022-detection-of-tbe-virus-in-dairy-products\/","title":{"rendered":"Snapshot week 23\/2022 <br>Detection of TBE virus in dairy products"},"content":{"rendered":"<span class=\"cb-itemprop\" itemprop=\"reviewBody\">\n<p class=\"wp-block-paragraph\">Hennechart-Collette et al.<br>Method for tick-borne encephalitis virus detection in raw milk products<br><em>Food Microbiol.<\/em> 2022;104:104003. doi:10.1016\/j.fm.2022.104003<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">TBE virus is most commonly transmitted by tick bites, but occasionally alimentary infections occur via the consumption of non-pasteurized milk or milk products. Recently, such a severe alimentary TBE outbreak with many individuals affected happened in France in 2020 and has been discussed in the <a href=\"https:\/\/tbenews.com\/tbe\/alimentary-tbe-outbreak-in-eastern-france\/\">Newsletter May 2022<\/a>.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">In consequence of this outbreak, a sensitive and reliable RT-qPCR-based method has been developed to detect TBE virus in dairy products based on ISO 15216, which is a method for the detection of enteroviruses (noroviruses and hepatitis A virus) in high-risk food categories.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">To detect the Limit of Detection (LOD), the extraction of TBE virus specific RNA, and for control of a murine norovirus strain from artificially contaminated milk and cheese, was determined, and RT-qPCR inhibitors were assessed.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">In this study, the LOD<sub>95<\/sub> value for TBE virus was 2.84 x 10<sup>4<\/sup> genome copies per g or per ml. The molecular method described in this study fulfilled the criteria to detect TBE virus in food according to ISO 15216 and could thus be used in routine diagnostic laboratories and to enable potential health risks of non-pasteurized milk and milk products to be assessed.<\/p>\n<\/span>","protected":false},"excerpt":{"rendered":"<p>Hennechart-Collette et al.Method for tick-borne encephalitis virus detection in raw milk productsFood Microbiol. 2022;104:104003. doi:10.1016\/j.fm.2022.104003 TBE virus is most commonly transmitted by tick bites, but occasionally alimentary infections occur via the consumption of non-pasteurized milk or milk products. Recently, such a severe alimentary TBE outbreak with many individuals affected happened in France in 2020 and has been discussed in the Newsletter May 2022. In consequence of this outbreak, a sensitive and reliable RT-qPCR-based method has been developed to detect TBE virus in dairy products based on ISO 15216, which is a method for the detection of enteroviruses (noroviruses and hepatitis A virus) in high-risk food categories. To detect the Limit of Detection (LOD), the extraction of TBE virus specific RNA, and for control of a murine norovirus strain from artificially contaminated milk and cheese, was determined, and RT-qPCR inhibitors were assessed. In this study, the LOD95 value for TBE virus was 2.84 x 104 genome copies per g or per ml. The molecular method described in this study fulfilled the criteria to detect TBE virus in food according to ISO 15216 and could thus be used in routine diagnostic laboratories and to enable potential health risks of non-pasteurized milk and milk products to be assessed.<\/p>\n","protected":false},"author":1354,"featured_media":12864,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"footnotes":""},"categories":[18],"tags":[513,887,888,514,889,890],"coauthors":[581],"class_list":["post-19576","post","type-post","status-publish","format-standard","has-post-thumbnail","category-snapshot","tag-cheese","tag-contamination","tag-dairy-products","tag-milk","tag-process-control","tag-rt-qpcr-detection"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Snapshot week 23\/2022 Detection of TBE virus in dairy products - TBE Book<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/tbenews.com\/tbe\/snapshot-week-23-2022-detection-of-tbe-virus-in-dairy-products\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Snapshot week 23\/2022 Detection of TBE virus in dairy products - TBE Book\" \/>\n<meta property=\"og:description\" content=\"Hennechart-Collette et al.Method for tick-borne encephalitis virus detection in raw milk productsFood Microbiol. 2022;104:104003. doi:10.1016\/j.fm.2022.104003 TBE virus is most commonly transmitted by tick bites, but occasionally alimentary infections occur via the consumption of non-pasteurized milk or milk products. Recently, such a severe alimentary TBE outbreak with many individuals affected happened in France in 2020 and has been discussed in the Newsletter May 2022. In consequence of this outbreak, a sensitive and reliable RT-qPCR-based method has been developed to detect TBE virus in dairy products based on ISO 15216, which is a method for the detection of enteroviruses (noroviruses and hepatitis A virus) in high-risk food categories. To detect the Limit of Detection (LOD), the extraction of TBE virus specific RNA, and for control of a murine norovirus strain from artificially contaminated milk and cheese, was determined, and RT-qPCR inhibitors were assessed. In this study, the LOD95 value for TBE virus was 2.84 x 104 genome copies per g or per ml. The molecular method described in this study fulfilled the criteria to detect TBE virus in food according to ISO 15216 and could thus be used in routine diagnostic laboratories and to enable potential health risks of non-pasteurized milk and milk products to be assessed.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/tbenews.com\/tbe\/snapshot-week-23-2022-detection-of-tbe-virus-in-dairy-products\/\" \/>\n<meta property=\"og:site_name\" content=\"TBE Book\" \/>\n<meta property=\"article:published_time\" content=\"2022-06-06T04:52:50+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2022-06-06T04:52:51+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/tbenews.com\/tbe\/wp-content\/uploads\/2017\/11\/TBE-Snapshots.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1180\" \/>\n\t<meta property=\"og:image:height\" content=\"250\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Shaqeez\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Shaqeez\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-23-2022-detection-of-tbe-virus-in-dairy-products\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-23-2022-detection-of-tbe-virus-in-dairy-products\\\/\"},\"author\":{\"name\":\"Shaqeez\",\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/#\\\/schema\\\/person\\\/2bc2ab1f56f93257537a0ce8de903224\"},\"headline\":\"Snapshot week 23\\\/2022 Detection of TBE virus in dairy products\",\"datePublished\":\"2022-06-06T04:52:50+00:00\",\"dateModified\":\"2022-06-06T04:52:51+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-23-2022-detection-of-tbe-virus-in-dairy-products\\\/\"},\"wordCount\":211,\"publisher\":{\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-23-2022-detection-of-tbe-virus-in-dairy-products\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/wp-content\\\/uploads\\\/2017\\\/11\\\/TBE-Snapshots.jpg\",\"keywords\":[\"cheese\",\"contamination\",\"dairy products\",\"milk\",\"process control\",\"RT-qPCR detection\"],\"articleSection\":[\"Snapshot\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-23-2022-detection-of-tbe-virus-in-dairy-products\\\/\",\"url\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-23-2022-detection-of-tbe-virus-in-dairy-products\\\/\",\"name\":\"Snapshot week 23\\\/2022 Detection of TBE virus in dairy products - 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