{"id":20846,"date":"2023-07-24T12:46:14","date_gmt":"2023-07-24T04:46:14","guid":{"rendered":"https:\/\/tbenews.com\/tbe\/?p=20846"},"modified":"2023-07-24T12:46:15","modified_gmt":"2023-07-24T04:46:15","slug":"snapshot-week-30-2023-virus-like-particle-based-vaccine-to-protect-against-tbe-virus-infection","status":"publish","type":"post","link":"https:\/\/tbenews.com\/tbe\/snapshot-week-30-2023-virus-like-particle-based-vaccine-to-protect-against-tbe-virus-infection\/","title":{"rendered":"Snapshot week 30\/2023
Virus-like particle-based vaccine to protect against TBE virus infection"},"content":{"rendered":"\n

Tang et al.
Development of a novel virus-like particle-based vaccine for preventing tick-borne encephalitis virus infection
Virol Sin<\/em>. 2023;S1995-820X(23)00072-X. Published online June 14, 2023. doi:10.1016\/j.virs.2023.06.003<\/p>\n\n\n\n

There are several TBE vaccines that have been approved and are highly effective. In spite of this, vaccine breakthrough infections are reported in a very small percentage of individuals who are fully vaccinated. Thus, alternative TBE vaccine research is ongoing, and a Chinese team has recently examined the immunogenicity of recombinant TBE virus-like particles (VLP).<\/p>\n\n\n\n

Plasmids were constructed coding for following TBE virus-specific proteins: core (C), pre-membrane glycoprotein (prM), glycoprotein E (E) and the non-structural proteins (NS2B\/NS3Pro) of the TBE-FE strain WH2012 and TBE-EU strain Neud\u00f6rfl. These proteins were expressed in Expi293F cells producing VLPs. The VLPs were purified by density-gradient sedimentation and had morphological characteristics similar to wild-type TBE virus particles with a diameter of 40\u201350 nm.<\/p>\n\n\n\n

Immunization of mice with VLPs induced a robust immune response (ELISA IgG and neutralizing antibodies), and the antiserum was able to neutralize both TBE-FE and TBE-EU virus infections in BHK-21 cells. Mice, immunized with VLPs, were protected against challenge with TBE virus and did not significantly lose weight, did not exhibit neurological symptoms, and viral loads in serum samples, intestinal tissue and brain were neglectable. In addition, it was shown that immunized mice produced anti-viral CD4+<\/sup> T cells producing TNF-a+<\/sup>, IL2+, or IFN-g+<\/sup>.<\/p>\n\n\n\n

These results showed the efficient construction of TBE VLPs by plasmid-driven transfection of viral proteins in mammalian cells and protection against TBE virus infection in mice.<\/p>\n<\/span>","protected":false},"excerpt":{"rendered":"

Tang et al.Development of a novel virus-like particle-based vaccine for preventing tick-borne encephalitis virus infectionVirol Sin. 2023;S1995-820X(23)00072-X. Published online June 14, 2023. doi:10.1016\/j.virs.2023.06.003 There are several TBE vaccines that have been approved and are highly effective. In spite of this, vaccine breakthrough infections are reported in a very small percentage of individuals who are fully vaccinated. Thus, alternative TBE vaccine research is ongoing, and a Chinese team has recently examined the immunogenicity of recombinant TBE virus-like particles (VLP). Plasmids were constructed coding for following TBE virus-specific proteins: core (C), pre-membrane glycoprotein (prM), glycoprotein E (E) and the non-structural proteins (NS2B\/NS3Pro) of the TBE-FE strain WH2012 and TBE-EU strain Neud\u00f6rfl. These proteins were expressed in Expi293F cells producing VLPs. The VLPs were purified by density-gradient sedimentation and had morphological characteristics similar to wild-type TBE virus particles with a diameter of 40\u201350 nm. Immunization of mice with VLPs induced a robust immune response (ELISA IgG and neutralizing antibodies), and the antiserum was able to neutralize both TBE-FE and TBE-EU virus infections in BHK-21 cells. Mice, immunized with VLPs, were protected against challenge with TBE virus and did not significantly lose weight, did not exhibit neurological symptoms, and viral loads in serum samples, intestinal tissue and brain were neglectable. In addition, it was shown that immunized mice produced anti-viral CD4+ T cells producing TNF-a+, IL2+, or IFN-g+. These results showed the efficient construction of TBE VLPs by plasmid-driven transfection of viral proteins in mammalian cells and protection against TBE virus infection in mice.<\/p>\n","protected":false},"author":1354,"featured_media":12864,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"footnotes":""},"categories":[18],"tags":[341,778,454,748,1026,249,1025],"coauthors":[581],"class_list":["post-20846","post","type-post","status-publish","format-standard","has-post-thumbnail","category-snapshot","tag-european-subtype","tag-far-eastern-subtype-2","tag-immunogenicity","tag-protection","tag-t-cell-response","tag-vaccine","tag-virus-like-particle"],"yoast_head":"\nSnapshot week 30\/2023 Virus-like particle-based vaccine to protect against TBE virus infection - TBE Book<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/tbenews.com\/tbe\/snapshot-week-30-2023-virus-like-particle-based-vaccine-to-protect-against-tbe-virus-infection\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Snapshot week 30\/2023 Virus-like particle-based vaccine to protect against TBE virus infection - TBE Book\" \/>\n<meta property=\"og:description\" content=\"Tang et al.Development of a novel virus-like particle-based vaccine for preventing tick-borne encephalitis virus infectionVirol Sin. 2023;S1995-820X(23)00072-X. Published online June 14, 2023. doi:10.1016\/j.virs.2023.06.003 There are several TBE vaccines that have been approved and are highly effective. In spite of this, vaccine breakthrough infections are reported in a very small percentage of individuals who are fully vaccinated. Thus, alternative TBE vaccine research is ongoing, and a Chinese team has recently examined the immunogenicity of recombinant TBE virus-like particles (VLP). Plasmids were constructed coding for following TBE virus-specific proteins: core (C), pre-membrane glycoprotein (prM), glycoprotein E (E) and the non-structural proteins (NS2B\/NS3Pro) of the TBE-FE strain WH2012 and TBE-EU strain Neud\u00f6rfl. These proteins were expressed in Expi293F cells producing VLPs. The VLPs were purified by density-gradient sedimentation and had morphological characteristics similar to wild-type TBE virus particles with a diameter of 40\u201350 nm. Immunization of mice with VLPs induced a robust immune response (ELISA IgG and neutralizing antibodies), and the antiserum was able to neutralize both TBE-FE and TBE-EU virus infections in BHK-21 cells. Mice, immunized with VLPs, were protected against challenge with TBE virus and did not significantly lose weight, did not exhibit neurological symptoms, and viral loads in serum samples, intestinal tissue and brain were neglectable. In addition, it was shown that immunized mice produced anti-viral CD4+ T cells producing TNF-a+, IL2+, or IFN-g+. These results showed the efficient construction of TBE VLPs by plasmid-driven transfection of viral proteins in mammalian cells and protection against TBE virus infection in mice.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/tbenews.com\/tbe\/snapshot-week-30-2023-virus-like-particle-based-vaccine-to-protect-against-tbe-virus-infection\/\" \/>\n<meta property=\"og:site_name\" content=\"TBE Book\" \/>\n<meta property=\"article:published_time\" content=\"2023-07-24T04:46:14+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-07-24T04:46:15+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/tbenews.com\/tbe\/wp-content\/uploads\/2017\/11\/TBE-Snapshots.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1180\" \/>\n\t<meta property=\"og:image:height\" content=\"250\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Shaqeez\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Shaqeez\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-30-2023-virus-like-particle-based-vaccine-to-protect-against-tbe-virus-infection\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-30-2023-virus-like-particle-based-vaccine-to-protect-against-tbe-virus-infection\\\/\"},\"author\":{\"name\":\"Shaqeez\",\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/#\\\/schema\\\/person\\\/2bc2ab1f56f93257537a0ce8de903224\"},\"headline\":\"Snapshot week 30\\\/2023 Virus-like particle-based vaccine to protect against TBE virus infection\",\"datePublished\":\"2023-07-24T04:46:14+00:00\",\"dateModified\":\"2023-07-24T04:46:15+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-30-2023-virus-like-particle-based-vaccine-to-protect-against-tbe-virus-infection\\\/\"},\"wordCount\":270,\"publisher\":{\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-30-2023-virus-like-particle-based-vaccine-to-protect-against-tbe-virus-infection\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/wp-content\\\/uploads\\\/2017\\\/11\\\/TBE-Snapshots.jpg\",\"keywords\":[\"European subtype\",\"Far-eastern subtype\",\"immunogenicity\",\"protection\",\"T cell response\",\"vaccine\",\"virus-like particle\"],\"articleSection\":[\"Snapshot\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-30-2023-virus-like-particle-based-vaccine-to-protect-against-tbe-virus-infection\\\/\",\"url\":\"https:\\\/\\\/tbenews.com\\\/tbe\\\/snapshot-week-30-2023-virus-like-particle-based-vaccine-to-protect-against-tbe-virus-infection\\\/\",\"name\":\"Snapshot week 30\\\/2023 Virus-like particle-based vaccine to protect against TBE virus infection - 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